Journal: iScience

Article Title: EFNB1 levels determine distinct drug response patterns guiding precision therapy for B-cell neoplasms

doi: 10.1016/j.isci.2023.108667

Figure Lengend Snippet:

Article Snippet: R x64 4.0.3 , , www.rstudio.com/.

Techniques: Virus, Recombinant, Mass Spectrometry, Western Blot, Retroviral, Plasmid Preparation, Software

Journal: Blood Advances

Article Title: Proteomic and bioinformatic profiling of neutrophils in CLL reveals functional defects that predispose to bacterial infections

doi: 10.1182/bloodadvances.2020002949

Figure Lengend Snippet: Key resource table

Article Snippet: FC measurement was performed on a BD LSR Fortessa II or BD Aria III, and data were analyzed with FlowJo 10 software. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Reagent or resource Source Identifier, catalog/stock no. Antibodies CD5 eBioscience 45-0051-82 CD16 human BD Bioscience 557744 CD19 BioLegend 115539, 115507 CD45 BioLegend 103154 CD62L BioLegend 104437 CD62L human BD Bioscience 560440 CD66b human Beckmann Coulter IM0531U Chicken anti-GFP Aves GFP-1020 CXCR4 BioLegend 146511 CXCR4 human BioLegend 306506 ICAM-1 BioLegend 116105 ICAM-1 human eBioscience 17-0549-42 Ly6G BioLegend 127607 Reagents 2-Iodacetamide Merck 8.047.440.025 Ammonium hydrogen carbonate PanReac AppliChem A3583 0500 Bovine serum albumin GE Healthcare K45-001 Collagenase Sigma-Aldrich C2674 DAPI Life Technologies D1306 Dithiothreitol PanReac AppliChem A1101 0025 DNase I Sigma-Aldrich D4513-1VL Isoflurane Abbott NA LB medium Carl Roth GmbH X64. l -lysine Sigma-Aldrich L5626 Neutrophil isolation kit Miltenyi Biotec 130-097-658 Paraformaldehyde Sigma-Aldrich P6148 PBS Life Technologies 18912-014 pHrodo Green E coli BioParticles Conjugate for Phagocytosis Thermo Fisher Scientific P35366 Phosphate buffer Sigma-Aldrich 71500 [NaH 2 PO 4 + Na 2 HPO 4 ] Carl Roth GmbH P030.1 RapiGest SF surfactant Waters 186001861 Sodium periodate (NaIO 4 ) Carl Roth GmbH 2603.1 Sucrose Carl Roth GmbH 9097.1 Tissue-Tek OCT Sakura 4583 Trifluoroacetic acid VWR 85.049.001 Triton X-100 Carl Roth GmbH 3051.4 Trypsin Serva 37286 Experimental models: mouse strains C57BL/6 J Jackson Laboratories 000664 UPEC strain 536 gfp (O6:K15:H31) Engel et al 34 doi: 10.1128/IAI.00881-06 Software Adobe Illustrator CC 2020 Adobe RRID:SCR_010279 Fiji ImageJ RRID:SCR_003070 FlowJo 10 FlowJo RRID:SCR_008520 GSEA, version 4.0.3 Broad Institute RRID:SCR_003199 GraphPad Prism 8 GraphPad Software RRID:SCR_002798 R Project for Statistical Computing, version 3.5.1 https://cran.r-project.org/ RRID:SCR_001905 ZEN Digital Imaging for Light Microscopy, ZEN 2012 Zeiss RRID:SCR_013672 Open in a separate window NA, not available.

Techniques: Isolation, Software, Imaging, Light Microscopy